PNC-27 Fact Sheet
- Anti-Cancer Activity
- Tumor Suppression
- Cancer Cell Cytotoxicity
PNC-27 Peptide Research
PNC-27 is an anti-cancer peptide initially studied for its unique mechanism of inducing cancer cell cytotoxicity by binding to HDM-2 in the membranes of cancer cells. The peptide contains the HDM-2 binding domain of p53 and causes tumor cell death by membranolysis.
It has been shown to induce tumor cell necrosis in both solid and non-solid tissue tumor cells, including a poorly differentiated non-solid tissue human leukemia cell line. PNC-27 and its shorter analog peptide, PNC-28, selectively lyse the membranes of cancer cells, leading to the necrosis of a variety of cancer cell lines and primary human tumors, but not untransformed cells.
PNC-27 as an Anti-Cancer Peptide
PNC-27 is a 32-residue peptide with anti-cancer activity that selectively kills cancer cells without harming normal cells. It contains an HDM-2 binding domain and a cell-penetrating peptide (CPP) leader sequence. PNC-27 works by binding to HDM-2 associated with the plasma membrane of cancer cells, inducing the formation of pores and causing tumor cell lysis and necrosis. This mechanism is independent of p53 activity in the cells.
The peptide has demonstrated effectiveness against various cancer cell lines, including breast, ovarian, cervical, and leukemia cells. In some cases, PNC-27 has been shown to enhance the efficacy of other anti-cancer agents, such as rapamycin, methotrexate, and the peptide PNC-27, by substantially lowering their IC50 values for cancer cell killing. This suggests that PNC-27 could be a powerful adjunct agent in treating human cancers.
PNC-27’s anti-cancer activity is dependent on its interaction with membrane-bound HDM-2, a zinc finger protein and negative regulator of the p53 pathway, which is often overexpressed by cancer cells and implicated in cancer cell proliferation and survival. The peptide does not kill cancer cells but rather blocks their proliferation. In some studies, PNC-27 has demonstrated synergistic effects with other anti-cancer drugs, such as paclitaxel, in the treatment of ovarian cancer.
What is PNC-27?
PNC-27 is a synthetic anti-cancer peptide that contains an HDM-2 binding domain and a cell-penetrating peptide (CPP) leader sequence. It is designed to selectively target and destroy cancer cells by binding to HDM-2 associated with the plasma membrane of these cells, inducing the formation of pores, and causing tumor cell lysis and necrosis.
What is HDM-2?
HDM-2 is also known as human double minute 2 and is an oncoprotein that plays an important role in regulating the tumor suppressor p53. It is known to be uniquely expressed in the membranes of both solid and non-solid tumor cells, regardless of their p53 status. This specific expression and localization make HDM-2 an attractive therapeutic target for cancer treatment.
How Does PNC-27 Work?
PNC-27 works by binding to the HDM-2 protein expressed in the cancer cell membranes. This interaction results in the formation of transmembrane pores in cancer cells, but not in normal cells. The formation of these pores leads to tumor cell necrosis, a process that is independent of p53 activity in these cells.
The PNC-27 peptide is drawn to tumor cells and then activates tumor suppression genes, primarily P-53, which is only activated in cells under stress, like all cancer cells.
Does PNC-27 Kill Cancer Cells?
Yes, PNC-27 has been shown to kill cancer cells. It is selectively cytotoxic to cancer cells, inducing nearly 100 percent cell killing with lactate dehydrogenase (LDH) release, a marker of cell necrosis. This effect has been observed in various types of cancer cells, including solid tissue tumor cells and non-solid tissue tumor cells like leukemia cells.
Does PNC-27 Induce Tumor Necrosis?
Yes, PNC-27 induces tumor necrosis. It does this by binding to the HDM-2 protein in the membranes of cancer cells, leading to the formation of transmembrane pores. The formation of these pores results in the death of the cancer cells, a process known as necrosis.
Where to Buy PNC-27 Anti-Cancer Peptide?
You can buy research peptides like PNC-27 from reputable online sources like Limitless Life Nootropics. However, it’s important to note that PNC-27 is currently not approved for use in the United States and is strictly intended for research purposes for in-vitro clinical studies only.